Zerina Hadžić1, Ivan Puhar2, *


1Department of Dentistry, Sarajevo School of Science and Technology, Sarajevo, Bosnia and Herzegovina - 2Department of Periodontology, School of Dental Medicine, University of Zagreb, Zagreb, Croatia


Introduction: The effects of smoking on the values of CRP, 8-0HdG, SOD, and MDA in saliva have not been sufficiently investigated. The aim of this study was to determine and analyze the levels of salivary CRP, SOD, MDA, and 8-OHdG in smokers and non-smokers with periodontitis stage III or IV before and after non-surgical periodontal therapy. 

Materials and Methods: Standard periodontal parameters and saliva samples were collected in 28 subjects with periodontitis stages III and IV. Three months after therapy, re-registration of periodontal parameters and re-sampling of saliva for analysis of salivary CRP, SOD, MDA, and 8-OHdG were done for all groups. 

Results: Statistical analysis revealed a statistically significant reduction of salivary CRP and oxidative stress parameters in groups I and II, 3 months after the therapy. CRP, 8-8-0HdG, and MDA were statistically significantly reduced in group III 3 months after supragingival periodontal therapy. Our study revealed decreased concentrations of salivary CRP 3 months after non-surgical periodontal therapy, with higher values of these parameters in smokers. 

Conclusion: The results of this study confirmed that parameters of oxidative stress 8-OHdG, MDA and SOD are sensitive to smoking and, although supragingival and subgingival full-mouth periodontal therapy has been performed, the values of these parameters are still higher in smokers compared to non-smokers with periodontitis. Further studies are needed to prove the reliability of salivary CRP, 8-0HdG, MDA, and SOD in the monitoring of periodontal disease. 


Periodontitis, C-reactive protein, malondialdehyde, superoxide dismutase, 8-Hydroxydeoxyguanosine.