YANRONG XIE,WENJUAN YU, FENGYUN YU, LEILEI BING
Department of Endocrinology, Yantai Hospital of Traditional Chinese Medicine, Yantai, China
PI3K/Akt signaling pathway is multi-functional, and a host of reports have proved a key role in regulating renal extracellular matrix accumulation in diabetic kidney disease. The present study aimed to investigate the protective effect of buckwheat flavonoids on diabetic nephropathy via PI3K/Akt signaling pathway. In the present study, a lot of experiments were performed to validate the above assumptions, such as H&E staining, RT-PCR, western blot, blood glucose, kidney index et al. From the experimental data, we found that buckwheat flavonoids could ameliorate complications of the rats bearing diabetic nephropathy. After 4 weeks of buck- wheat flavonoids administration, the mice exhibited an excellent improvement in blood glucose and kidney index, reduced morpho- logical changes in the renal tissue and normalized the abnormal expression of FOXO1 mRNA. Treatment of buckwheat flavonoids also down-regulated p-Akt and PI3K (p85) proteins level caused by diabetic nephropathy, which indicated the involvement of PI3K/Akt signaling pathway in the diabetic nephropathy. Our results verified buckwheat flavonoids as a potential therapeutic agent evidently by improving the renal function via inhibiting PI3K/Akt signaling pathway, consequently ameliorating diabetic nephropa- thy.
buckwheat flavonoid, diabetic nephropathy, PI3K/Akt signaling pathway, FOXO1 mRNA