Department of Neurology, Cangzhou Central Hospital, Hebei Province, 061000


Objective: The purpose of this study was to investigate the effect of THZ1 on proliferation and apoptosis of human glioma U373-MG cells.

Methods: The study involved three sets of cells namely, experimental group, blank control group and DMSO group. The exper- imental group was treated with THZ1 at different concentrations, and cell proliferation was determined by MTT assay, while cell cycle and apoptosis analyses were assessed by flow cytometry. The expression of caspase-3/7 was determined by Western blot.

Results: The rate of cell growth in the experimental group began to slow down from the third day in a time- and dose-depen- dent manner. The rate of growth of this group of cells was statistically significant when compared with blank control group and DMSO group (p < 0.05). Cell cycle determination showed that THZ1 arrested U373-MG cells in G1 phase, with relatively small number of cells in the S phase. This observation suggests that THZ1 blocks the proliferation of tumor cells by inhibiting cell cycle progression. Besides, THZ1 promoted the apoptosis of U373-MG cells and it probably accomplished this by increasing the activity of caspase-3/7 related apoptosis.

Conclusion: Results from the present study indicate that THZ1 can inhibit the proliferation of human glioma U373-MG cells, block cell cycle progression and promote apoptosis.


THZ1, Glioma, Proliferation, Apoptosis