JING-LI LIU, XIAO-LIN GAO, CONG-ZHE HOU, LIN ZHU, HUI XU
Department of Obstetrics and Gynecology, The Second Hospital of Shandong University, Jinan City, Shandong Province, 250012, China
Objective: To study changes in serum human epididymis epithelial secretory protein 4(HE4)and carbohydrate antigen 125(CA125)in endometrial carcinoma (EC)patients, and the diagnostic significance of these changes.
Methods: A total of 40 cases of EC patients were assigned to the EC group, 20 patients with endometrial hyperplasia were in the endometrial hyperplasia group, while 30 healthy subjects were in the control group. Changes in HE4 and CA125 levels in the three groups were analyzed before and after operation using ELISA. Positive expressions and sensitivity indicators were used to determine efficiency and relationship between the changes in serum HE4 and CA125, and histological grades and surgical staging.
Results: Before operation, serum HE4 and CA125 levels in the EC group were significantly higher than those in the endome- trial hyperplasia and control groups (p < 0.05). Serum HE4 and CA125 levels of endometrial hyperplasia group were significantly higher than those of the control group (p < 0.05), and the sensitivity of the combination of HE4 and CA125 in the diagnosis of EC was significantly higher than that of either marker alone (p < 0.05). Serum HE4 and CA125 levels in EC 7 days after operation were significantly lower than those before operation (p < 0.05). Analyses of histological types showed that the highest positive expression of HE4 was pathological grade 3 (G3) while the lowest positive expression of HE4 was G1 (p < 0.05); G3 was significantly higher than G2 (p < 0.05). In analyses of surgical stages, the highest positive expressions of HE4 and CA125 were stage III, and the lowest were stage I (p < 0.05); stage III was significantly higher than stageII(p < 0.05). Serum HE4 and CA125 levels in the recurrence group at 18 and 24 months after operation were significantly higher than those in non-recurrence group (p < 0.05).
Conclusion: Combined determination of serum HE4 and CA125 in the diagnosis of EC has high sensitivity. This has important clinical value for early diagnosis, and is useful for guiding operation and prognosis.
Endometrial carcinoma, Serum human epididymal epithelial secretory protein 4, Carbohydrate antigen 125, Histological types