Junsong Song, Borong Leng, Xuejun He*, Bingfeng Li, Rui Wang


College of Life and Health, Nanjing Polytechnic Institute, Nanjing, 210000, China


In order to investigate the mechanism of action of HAP nanoparticles on HeLa cells, the nano solution was prepared by HAP nanoparticles and mixed with the anticancer drug nicotine. The apoptosis degree of HeLa cells was detected using in situ terminal labeling method. The experimental data of each group in the experiment were analyzed by State7.0 software, and the mechanism of action of HAP nanoparticles on HeLa cells was investigated. The results showed that the nanoparticles with a diameter of 56.8nm could not only pass through the cytoplasm but also enter the nucleus. Therefore, HAP nanoparticles with a smaller diameter could enter the nucleus of HeLa cells by targeting and releasing the active anticancer drugs into the nucleus of HeLa cells to eliminate them. HeLa cells were induced by HAP nanoparticles. There was a strong dependence on the dose and time of HeLa cells and HAP nanoparticles. It could be concluded that reasonable selection of drug concentration and time could make HeLa cells exert a satisfactory apoptotic effect. SFSP significantly inhibited the proliferation of HeLa cells, and SFSP had a strong cytotoxic effect on HeLa cells.


TdT-mediated dUTP-biotin nick end labeling, apoptosis, HAP nanoparticles, HeLa cells, mechanism of action.