Authors

Yue Fang, Chenglong Lou, Yangping Ding, Liqin Liu*


Departments

Department of Chinese Pharmacy, Hangzhou Red Cross Hospital, Hangzhou, PR China

Abstract

Objective: To investigate the protective effect of resveratrol (Res) on the liver by regulating the signaling pathway of apoptosis and silencing regulator 1 (SIRT1)/high-mobility group protein B1 (HMGB1). 

Methods: 6-week-old male SD rats (48) were randomly divided into Sham, Hepatectomy and Res treatment groups, with 16 rats in each group. In the Sham group, simple abdominal dissection and extrahepatic vascular separation were performed, and 1mL·kg-1, 1d/1 of normal saline was intraperitoneally injected at 1w before surgery, until the end point of observation. Rats in the Hepatectomy group underwent partial hepatectomy, and the remaining procedures were the same as those in the Sham group. All rats in the Res group underwent partial hepatectomy, and Res 30mg·kg-1 was intraperitoneally injected at the same time at 1w before surgery, for 1d/time, until the end point of observation. The apoptosis number, acetylated HMGB1 and SIRT1 protein expression levels, serum HMGB1 levels and serum HMGB1 levels of rats in each group were compared after hepatectomy. 

Results: The number of apoptosis of hepatocytes in the Hepatectomy group was significantly higher than that in the Sham group (P<0.05). Moreover, the number of apoptosis of hepatocytes in the Res group was significantly lower than that in the Hepatectomy group (P<0.05). At 30min, 24, 48 and 72h, the HMGB1 level of rats in the Hepatectomy group was significantly higher than that in the Sham group (P<0.05). The HMGB1 levels of rats in the Res group were significantly lower than those in the Sham group (P<0.05). The expression level of acetylated HMGB1 protein in the rat liver tissues of the Hepatectomy group was significantly higher than that of the Sham group, and the expression level of SIRT1 protein was significantly lower than that of the Sham group. However, the expression level of acetylated HMGB1 protein in the liver tissue of rats in the Res group was significantly lower than that in the Hepatectomy group, and the expression level of SIRT1 protein was significantly higher than that in the Hepatectomy group (P<0.05). 

Conclusion: Resveratrol may play a protective role in the liver by inhibiting apoptosis, possibly through the SIRTI/HMGB1 signaling pathway. 

 

Keywords

Hepatocellular carcinoma, resveratrol, apoptosis, SIRTI, HMGB1.

DOI:

10.19193/0393-6384_2021_3_266