CLINICAL SIGNIFICANCE OF SERPINA3 IN LIVER CANCER AND ITS EFFECT ON PROLIFERATION AND INVASION OF CANCER CELLS

Zhangdong Feng, Wei Han^*

Department of General surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China

Objective: This study aimed to explore the clinical significance of SERPINA3 in liver cancer (LC) and its effect on proliferation and invasion of cancer cells. 

Methods: Eighty cases of tumor tissue and 80 cases of paracancerous tissue cut from hepatocellular carcinoma (HCC) patients from March 2016 to March 2019 were selected as research samples. Meanwhile, human LC Bel-7402 cells were transfected and SERPINA3 expression was knocked down. The SERPINA3 expression in cells of tumor tissues and paracancerous tissues was detected, and its expression levels in different clinical features were compared. The SERPINA3 expression levels of cells in the two groups after transfection were detected, so did cell proliferation, invasion and apoptosis, and epithelial-mesenchymal transition (EMT). Then, the risk and prognosis factors were analyzed. 

Results: The SERPINA3 expression level was higher in tumor tissues, and the levels of larger tumor diameter and later stage were higher. After transfection, the SERPINA3 expression level in the si-SERPINA3 group was lower, while its invasion and proliferation levels were lower, apoptosis rate was higher, and Snail and Twist1 levels were lower. SERPINA3, Snail and Twist1, as well as tumor diameter and pathological stage of cancer can be used as prognostic factors. 

Conclusion: SERPINA3 accelerates the proliferation of cancer cells and increases the tumor volume, developing LC to advanced stage and accelerating the invasion of cancer cells.