Ruicai Yang*, Yinsheng Li***, Shanshan Kong****, Yanan Zhang*****, Chengrui Li**, #


*Henan College of Industry & Information Technology, Jiaozuo City, Henan Province, China - **Department of Anesthesiology, Lianshui People's Hospital, Huaian City, Jiangsu Province, China - ***School of Pharmacy, Sanquan College of Xinxiang Medical University, Xinxiang City, Henan Province, China - ****Department of Medicine and Equipment, Henan Architectural Hospital, Zhengzhou City, Henan Province, China - *****Department of Pharmacy, Wuyang People’s Hospital, Luohe City, Province, China


Objective: To study the effect of propofol on hepatoma MHCC97 cells and its possible regulatory mechanisms. 

Methods: Real-time quantitative PCR was used to test the expression of miR-21 in hepatocellular carcinoma cells. After treatment with propofolum, the effect of miR-21 on the proliferation of hepatoma cells was determined by CCK-8 method, the cell apoptosis was analysed by flow cytometry, and the survival-related proteins, apoptosis-related proteins and EMT-related proteins that were also detected by immunofluorescence staining were detected by Western blot. 

Results: It was found that propofol could inhibit the expression of miR-21, and after its expression decreased, the proliferation of MHCC97 cells decreased and apoptosis significantly increased. At the same time, propofol reduced the expression of MHCC97 EMT. Furthermore, propofol stimulated the pathways activity of PI3K/AKT and Wnt3a/β-catenin reduced that miR-21dependent. 

Conclusion: Propofol can not only inhibit the proliferation of MHCC97 cells and decreased EMT by down-regulating the expression of miR-21 to promote apoptosis, but it can also further regulate the pathway of PI3K/AKT and Wnt/β-catenin.


miR-21, hepatoma, EMT, propofol.