Authors

Yue Yu*, Haibo Xi*, Xiaokang Zhu**, Gongxian Wang*, #

Departments

*Department of Urology, First affiliated hospital of Nanchang university, Nanchang, PR China -**School of Nanchang University, Nanchang, PR China

Abstract

Objective: To measure the expression of Tamm-Harsefall (TH) protein and osteopontin (OPN) in the urine of patients with urinary calculus and its predictive value. 

Methods: Fifty-five patients with urinary calculi treated within the urology department in our hospital from May 2017 to May 2018 were selected as the observation group, and 55 healthy people who attended the hospital for physical examination were selected as the control group. The levels of urinary crystals (urinary calcium, urinary phosphorus, oxalic acid, uric acid, citric acid, calcium oxalate and calcium phosphate) were compared across the two groups. The levels of urinary TH protein and OPN were measured by enzyme-linked immunosorbent assay (ELISA). Logistic regression was used to analyse the risk factors affecting urinary calculi. We conducted a Receiving Operating Characteristic (ROC) curve analysis of the value of urinary TH protein and OPN alone and combined as predictors of urinary calculi. 

Results: Phosphoric acid, uric acid, calcium oxalate and calcium phosphate were significantly higher in the observation group than in the control group (P<.05), and citrate was significantly lower than the control group (P<.05). The levels of urinary TH protein and OPN in the observation group were significantly lower than those in the control group, and the difference was statistically significant (P<.05). Logistic regression analysis showed that the decrease in TH protein and OPN levels in urine and the increase in calcium oxalate and calcium phosphate levels were risk factors for urinary calculi (P<.05). Urinary TH protein and OPN individually predicted the area under the curve (AUC) of urinary calculi, at 0.783 and 0.826, respectively. The sensitivity for urinary TH protein and OPN was 80.08% and 85.36%, respectively, and the specificity was 75.22% and 81.78%. The two indicators combined predicted urinary calculi with an AUC of 0.926 and sensitivity and specificity of 89.65% and 94.73%. Compared with the detection of urinary TH and OPN alone, the detection of both proteins had a higher predictive value. 

Conclusions: Urinary TH protein and OPN were found in the urine of patients with urinary calculi, and the two indicators are risk factors for urinary calculi. The detection of both urinary TH protein and OPN has high clinical value for predicting urinary calculi.

Keywords

Tamm-Harsefall protein, osteopontin, urinary calculus, joint prediction, value.

DOI:

10.19193/0393-6384_2020_3_223