Yong Hu, Honghai Wang, Jun Zhang, Jun Wu#


Department of Spinal Surgery, Changzhou First People's Hospital, Changzhou, Jiangsu


Objective: The purpose of this study was to explore the relationship between microRNA-218, survival hormone and its mechanism in osteosarcoma. 

Methods: The transcription levels of microRNA-218 and survivin in osteosarcoma cells MG63 and U2OS and human osteoblast cell line hFOB1.19 were measured by quantitative RT-PCR, and the protein expression of survivin and related apoptotic proteins Caspase-3 and Caspase-9 were detected by western blotting. The effect of microRNA-218 on cell proliferation was evaluated by a cell counting kit, and the effect of microRNA-218 on cell migration and invasion was investigated by Transwell assay. The interaction and mechanism between microRNA-218 and the survivor were studied by qRT-PCR, western blotting and luciferase assay. 

Results: RT-qPCR results showed that microRNA-218 was downregulated in osteosarcoma cell lines MG63 and U2OS compared with human osteoblast cell line hFOB1.19. Western blotting and PCR results showed that compared with hFOB1.19 cells, the expression of survivin in MG63 and U2OS cells was significantly increased. They were negatively correlated with each other, while the expression of apoptosis proteins Caspase-3 and Caspase-9 was significantly decreased. The binding sequence of survivor to microRNA-218 was predicted by Targetscan, and the results of double luciferase assay showed that the survivor was the direct target of microRNA-218. The overexpression of microRNA-218 significantly decreased the proliferation, migration and invasion of osteosarcoma cells. The expression of the survivor decreased, while the overexpression of microRNA-218 blocked the inhibitory effect of the upregulation of microRNA-218 on the proliferation and invasion of osteosarcoma cells. 

Conclusion: Our data show that microRNA-218 affects osteosarcoma cells by regulating the expression of survivin, which may provide a theoretical basis for the treatment of osteosarcoma with microRNA-218. 


MicroRNA-218, survivor, osteosarcoma.