Authors

Zhongwei Wu*, Yinyu Zhang**, #, Chaoquan Liu*, Shengji Zhao*

Departments

*Department of Cardiology, Hainan Western Central Hospital, Danzhou, PR China - **Department of Cardiovascular Medicine, Zhuji People's Hospital, Zhuji, PR China

Abstract

Objective: To investigate the mechanism of insulin in down-regulating the expression of UCP2 in damaged cardiomyocytes by up-regulating the expression of LPS-induced H9C2 cardiomyocytes.

Methods: H9c2 cells were randomly divided into the control group, the LPS group, IN 70 IU/L group, IN 350 IU/L group, and IN 700IU/L group. At 15 minutes prior to LPS stimulation, IN 70 IU/L group, IN 350 IU/L group and IN 700IU/L group were treated with the same amount of 9g/L saline as the LPS group with the corresponding dose of IN, control group. The changes of lactic dehydrogenase (LDH), cell activity, malondialdehyde (MDA) (MDA) and (SOD), activity oxygen cluster (ROS), uncoupling protein 2 (UCP2), tumour necrosis factor α (TNF- α) and IL-1 β (IL-1 β) in each group were then compared.

Results: The level of LDH in the LPS group was significantly higher, and the cell activity was significantly lower, than that of the control group (P<0.05). The LDH level in LDH group was significantly lower than that of the LPS group, and the cell activity in LPS group was significantly higher than that of the LPS group (P < 0.05). The level of LDH in the IN 350IU/L group was significantly lower than in the IN 700IU/L group, while the cell activity was significantly higher than that of the IN 700IU/L group (P<0.05). The cell activity in IN 700IU/L group was significantly lower than that in control group (P<0.05); IN 350IU/L). In addition, the level of the LDH in the IN 700IU/L group was significantly lower than that in LPS group, and the cell activity in IN 700IU/L group was significantly higher than in the LPS group (P<0.05). The level of LDH in IN 350IU/L group was significantly lower than that in the IN 700IU/L group, and the cell activity was significantly higher than that found in the IN 700IU/L group (P<0.05). The expression of UCP2 protein and UCP2mRNA in). The LPS group was significantly higher than that of the control group (P<0.05). The expression of UCP2 protein and UCP2mRNA in IN 350 IU/L and IN 700IU/L group was significantly higher than that in LPS group (P<0.05), and the insulin level in IN 350 IU/L group was found to be the best (P<0.05). The level of TNF- α in). LPS group was significantly higher than that in control group (P<0.05); IN 350 IU/L, IN 700IU/L group, P<0.05). The level of TNF-α in IN 350 IU/L group was significantly lower than that in the IN 70 IU group, and the level of IL-1 β in IN 700IU/L group was significantly higher than that of the control group (P < 0.05); IN 70IU/L group, IN 350 IU/L group, IN 700IU/L group and LPS group). 

Conclusion: IN may inhibit the release of ROS by up-regulating the expression of UCP2 in damaged cardiomyocytes and alleviate the inflammatory response and oxidative stress of H9c2 cardiomyocytes that is induced by LPS.

Keywords

Insulin, sepsis, H9c2 cells, LPS.

DOI:

10.19193/0393-6384_2020_3_235