Authors

Qin Shen#, Dianfu Li, Yanrong Liu, Chunping Tang, Yingming Zhao, Zemu Wang

Departments

Department of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, PR China

Abstract

Objective: To study the expression level of IL-12p35 gene in a hypertensive cardiac hypertrophy mouse model and its effect on JAK2/STAT4 signalling pathway. 

Methods: Wild-type C57BL/6J mice were divided into a negative control group (6 rats) and a model group (6 rats). IL-12p35-KO mice were divided into an experimental group (6 rats) and a conditional control group (6 rats). The hypertensive cardiac hypertrophy mouse model was induced and established by Ang-II, and the changes of IL-12p35 mRNA and indicators related to cardiac hypertrophy were detected by real-time quantitative PCR. The degree of myocardial fibrosis was detected by Masson staining. The expression of myocardial protein was detected by immunoblotting. 

Results: The IL-12p35 mRNA level in the model group was significantly higher than in the negative control group (P<0.05). The ANP mRNA, BNP mRNA and Collagen I mRNA levels in the experimental group were significantly higher than in the negative control group (P<0.05). The level of Collagen I mRNA in the model group was significantly higher than in the negative control group (P<0.05). The ANP mRNA and Collagen I mRNA levels in the conditional control group were significantly higher than those in the negative control group (P<0.05). Masson staining showed that the degree of myocardial fibrosis in the experimental group was significantly higher than in the negative control group, the model group and the conditional control group (P<0.05). Immunoblotting showed that the expressions of P62 and LC3 I/II in the model group, experimental group and conditional control group were significantly higher than in the negative control group (P<0.05). The phosphorylation of JAK2 and STAT4 pathways in the experimental group and the control group was significantly higher than in the negative control group (P<0.05). The phosphorylation of the STAT4 pathway in the experimental group was significantly higher than in the model group, and the difference was statistically significance (P<0.05). 

Conclusion: The expression of IL-12p35 gene in myocardial tissue of hypertensive cardiac hypertrophy mice was significantly increased, which had the effect of inhibiting inflammation and regulating autophagy. This may be achieved by mediating the JAK2/STAT4 signalling pathway. 

Keywords

Hypertensive cardiac hypertrophy, IL-12p35 gene, JAK2/STAT4 signalling pathway.

DOI:

10.19193/0393-6384_2020_3_314