Xuezhi Luo*, Yudong Zheng*, Haibo Ding**, Wenyi Lu*, Lian Liu*, Jiangrong Huang*,#, Wei Huang***,#, Yufeng Yang****,#


*Medical School of Yangtze University, Jingzhou, Hubei,434023, PR China - **Tianjin Hospital ,Tianjin 300211,PR China - ***Department of Endocrinology, Jingzhou Hospital of Traditional Chinese Medicine, Third Clinical Medical College, Yangtze University, Jingzhou 434023, PR China - ****Experimental Teaching center,Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, 110847, PR China


Background: The effects of aqueous extract of Potentilla discolor combined with metformin on glucose and lipid metabolism and islet function in type 2 diabetic rats were observed.

Methods: Type 2 diabetes rat models were induced by fat emulsion formulation and small dose of streptozocin. Biochemical assay was applied to measure serum glucose,total cholesterol (TC) ,high density lipoprotein (HDL), low density lipoprotein (LDL) and triglycerides (TG). ELISA was applied to measure serum insulin levels. Pancreatic histology was examined by HE staining. Immunohistochemistry (IHC) was employed to examine Forkhead box O1 (FoxO1), p-FoxO1 and insulin protein expression.

Results: Compared with metformin alone, the aqueous extract of Potentilla discolor combined with metformin can groups significantly decreased random blood glucose and TC levels, increased insulin expression, promoted the release of insulin, decreased the expression of FoxO1 protein in the nucleus, induced the phosphorylation of FoxO1 protein and its getting out of the nucleus.

Conclusion: It suggested that the treatment of T2DM with the water extract of potentilla discolor combined with metformin may be better for the regulation of FoxO1 and its phosphorylation, the regulation of insulin release, the improvement of beta cell growth, and the protection of pancreas. Integrative Chinese and Western medicine can reduce the side effects of western medicine, protect and repair islet cells, and increase insulin sensitivity.


Pancreas, Potentilla discolor aqueous extract, Type 2 diabetes, FoxO1.