Authors

Hongyu Xue*, Huiying Wang**, #, Hongyi Zhao***, Song Zhang*

Departments

*Department of Plastic Surgery, Peking University Third Hospital, Beijing, PR China - **Department of Burns and Plastic Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, PR China - ***Department of Plastic Surgery, Beijing Hospital, National Center of Gerontology, Beijing, PR China

Abstract

Objective: To investigate the effects of 1,6-fructose diphosphate on glycolipid metabolism of adipocytes and angiogenesis in the graft.

Methods: Free fat was obtained and treated. FBP was dissolved in normal saline and divided into 4 mg / g, 2.5 mg/g,1 mg/g,0.5 mg/g FBP injection group and normal saline group was selected as control. There were 5 mice in each group at each time point. Mice were given intraabdominal injection 2 hours before operation and continuous intraperitoneal administration for 6 days after operation. HE staining, eight-factor immunofluorescence staining and real-time fluorescence quantitative PCR were used to analyze the effects of FBP on lipid metabolism of adipocytes and angiogenesis in the graft.

Results: For 2 weeks, 12 weeks after transplantation, the number of survival adipocytes in 2.5 mg/g, 4mg/g FBP injection group was significantly higher than that in control group (P<0.05). The intercellular profile was clear and full in the FBP injection group, but the cells in control group had more damage. From 2 weeks after transplantation, GPDH in 4mg/g FBP injection group was significantly higher than that in control group (P<0.05). At 2 weeks after transplantation, the level of PPAP-γ in 4 mg / g FBP injection group was significantly higher than that in control group (P<0.05), but there was no significant difference between the two groups after 8 weeks (P>0.05). At 2 weeks after transplantation, the number of blood vessels in 4 mg / g FBP injection group was remarkably higher than that in control group (P<0.05), but there was no significant difference in the number of blood vessels among the groups at 12 weeks after transplantation (P>0.05). At 2 weeks after transplantation, the expression of vascular endothelial cells VEGF in 4 mg / g FBP injection group was markedly higher than that in control group (P<0.05).

Conclusion: FBP can protect the key enzymes of glycolipid metabolism of adipocytes in grafts, maintain the normal function of cells, and promote the formation of blood vessels.

Keywords

FBP, adipocytes in grafts, AFP, glycolipid metabolism, angiogenesis.

DOI:

10.19193/0393-6384_2020_3_305