Authors

Hongyu Xue*, Huiying Wang**, #, Hongyi Zhao***, Song Zhang*

Departments

*Department of Plastic Surgery, Peking University Third Hospital, Beijing, PR China - **Department of Burns and Plastic Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, PR China - ***Department of Plastic Surgery, Beijing Hospital, National Center of Gerontology, Beijing, PR China

Abstract

Objective: To investigate the effects of 1,6-fructose diphosphate (FBP) on the glycolipid metabolism of adipocytes and angiogenesis in grafts.

Methods: Free fat was obtained and treated. FBP was dissolved in normal saline and divided into 4 mg/g, 2.5 mg/g, 1 mg/g and 0.5 mg/g FBP injection groups, and a normal saline group was selected as the control group. There were five mice in each group at each time point. Mice were given an intraabdominal injection 2 hours before the operation and continuous intraperitoneal administration for 6 days after the operation. Hematoxylin and eosin (H&E) staining, eight-factor immunofluorescence staining and real-time fluorescence quantitative polymerase chain reaction (PCR) were used to analyse the effects of FBP on the lipid metabolism of adipocytes and angiogenesis in grafts.

Results: For 2 weeks, 12 weeks after transplantation, the number of survival adipocytes in the 2.5 mg/g and 4 mg/g FBP injection groups was significantly higher than that in the control group (P<0.05). The intercellular profile was clear and full in the FBP injection groups, but the cells in the control group had more damage. From 2 weeks after transplantation, 3-phosphate glycerol dehydrogenase (GPDH) in the 4 mg/g FBP injection group was significantly higher than that in the control group (P<0.05). At 2 weeks after transplantation, the level of activated receptor γ (PPAP-γ) in the 4 mg/g FBP injection group was significantly higher than that in the control group (P<0.05), but there was no significant difference between the two groups after 8 weeks (P>0.05). At 2 weeks after transplantation, the number of blood vessels in the 4 mg/g FBP injection group was remarkably higher than that in the control group (P<0.05), but there was no significant difference in the number of blood vessels among the groups at 12 weeks after transplantation (P>0.05). At 2 weeks after transplantation, the expression of the cells’ vascular endothelial growth factor (VEGF) in the 4 mg/g FBP injection group was markedly higher than that in the control group (P<0.05).

Conclusion: FBP can protect the key enzymes of the glycolipid metabolism of adipocytes in grafts, maintain the normal function of cells and promote the formation of blood vessels.

Keywords

FBP, adipocytes in grafts, AFP, glycolipid metabolism, angiogenesis.

DOI:

10.19193/0393-6384_2020_3_210