Authors

Bo Zhang*, Chuanjin Sun**, Wen Gao***, Xiangwen Fan****,#

Departments

*Department of Anorectal, Zhongda Hospital Southeast University, Nanjing 210009, China - **Department of Nephrology, The People's Hospital of Zhangqiu Area, Jinan 250200, China -***Department of Acupuncture and Moxibustion, The People's Hospital of Zhangqiu Area, Jinan 250200, China - ****Department of Oncology Surgery, Baogang Hospital of Inner Mongolia, Baotou 014010, China

Abstract

Objective: To investigate the effect and mechanism of miR-143-3p on the proliferation and apoptosis of colorectal cancer (CRC) cells.

Methods:  qRT-PCR and WB were used to detect the expression of miR-143-3p and sine-sphere related homeobox 4 (SIX4) in CRC cells HCT116, SW480, DLD-1, COLO201 and normal colon tissue cells CCD-33Co. -143-3p-mimics, miR negative control (miR-NC), targeted inhibition of SIX4 RNA (si-SIX4), targeted overexpression of SIX4 RNA (sh-SIX4), negative control RNA (Si-NC), transfection Into HCT116 and SW480 cells. MTT, Transwell, and flow cytometry were used to detect cell proliferation, invasion, and apoptosis. The dual luciferin report confirmed the relationship between miR-143-3p and SATB.

Results: miR-143-3p was under expressed in CRC cells, while SIX4 expression was reversed. Cell experiments found that up-regulating the expression of miR-143-3p can inhibit the proliferation and invasion of CRC cells, promote apoptosis, and significantly reduce the expression of SIX4. Inhibition of SIX4 expression can inhibit cell proliferation and invasion and promote cell apoptosis. The double luciferin report identified a targeted regulatory relationship between miR-143-3p and SATB.

Conclusion: miR-143-3p can inhibit the proliferation and invasion of CRC cells and promote cell apoptosis through targeted regulation of SIX4, which is expected to become a potential clinical therapeutic target.

Keywords

miR-143-3p, colorectal cancer, biological function, SIX4.

DOI:

10.19193/0393-6384_2020_3_263