Zhao Wenjing*,#, Zhai Feihong*, Xu Nuo*, Wang Qi**


*Department of Biology, Taiyuan Normal University, Jinzhong, China - **School of Life Science, Shanxi University, Taiyuan, China


Objective: To explore the cellular response mechanisms of PT95 strain to cAMP and their application in the treatment of diabetes and obesity. 

Methods: Potato glucose medium (PDB) was used as the basic medium, and cAMP was added in concentrations of 0.01, 0.03, and 0.05 mmol/mL. The mycelia of Penicillium thomii PT95 on different media were collected. Carotenoid content, lipid peroxidation level, antioxidant enzyme activity, and antioxidant activity of the strains under different culture conditions were determined. The culture with a cAMP concentration of 0.03 mmol/mL was selected for transcriptome sequencing, and the differentially expressed genes (SOD and MAPK) were screened. The target genes were verified by fluorescence quantitative PCR, and the gene expression was significantly up-regulated.

Results: Carotenoid content, lipid peroxidation level and antioxidant index were positively correlated, while the activity of antioxidant enzymes was negatively correlated with cAMP content. With the same cAMP content, the total phenol content, DPPH free radical scavenging effect, and CAT activity increased with the increase of culture time, while lipid peroxidation level and SOD activity decreased. Carotenoid content reached the highest level after 5 days of culture, and the addition of 0.03 mmol/mL cAMP was able to significantly up-regulate the gene expression of PT95 strain: expression of the SOD gene was 25.3076 times higher (P <0.05) and expression of the MAPK gene was 20.9184 times higher (P <0.05) than before. 

Conclusion: This study showed that the development of PT95 strain was accompanied by oxidative stress, and a series of metabolic changes were observed, which resulted in the up-regulation of differentially significant genes. These results may contribute to the treatment of colon cancer.


Penicillium thomii, sclerotium, oxidative stress, cell response, colon cancer.