DISTRIBUTION AND GENE DETECTION OF DRUG-RESISTANT BACTERIA IN THE AIR OF HOSPITAL

YAN XU, HUIZHEN DING, WUFENG#

The Affliliation Hospital of Yangzhou University Respiratory Department. No.45 Taizhou road, Yangzhou city

Purpose: At the environmental level, we should monitor the resistance genes in the air to control the occurrence of nosocomial infections. 2. The distribution of pathogenic microbes in different sections and seasons is different, and the resistance genes of resistant bacteria in different sections and seasons are detected to know whether the resistance genes in each department are homologous, so as to monitor the role of resistant bacteria in the air. 3. Detection of antagonistic genes can be used to understand the resistance of resistant bacteria to antibiotics, to discover the variation of resistance genes in time, and to guide the drug use scheme of drug resistant bacteria in this area.

Methods: 1. Hospital air sample collection: 10 sampling points including the hospital corridor, emergency infusion, general outpatient, Department of respiration ward, neonatal ward, general paediatric ward, digestive ward, ICU ward, Department of cerebral surgery ward, urology ward, were used as the air medium sampling point, and the investigation of resistance gene pollution were conducted in four seasons: spring, summer, autumn and winter. 2. Qualitative and quantitative analysis of resistance genes: the target samples were collected for qualitative and quantitative analysis of target genes in the laboratory.

Results: 1. All the air samples in the hospital environment were integrated to analyze the proportion and structure of the pathogenic microorganism in the hospital environment. The proportion of the number of pathogenic microbes in the hospital environment accounted for 3.64% of the total number of all the analytical samples. 2. In different seasons, the main pathogenic bacteria are different, and the drug-resistant bacteria are also different. In the general ward, ICU has different resistance genes. 3. The content of macrolide resistance gene was significantly higher than that of beta lactam and quinolone.

Conclusions: (1) the basic status of resistance gene pollution in our hospital: The distribution of resistant bacteria in the air of general departments of hospitals and their resistance genes are homologous, because of the change of the first five drug-resistant bacteria with the seasonal changes, so we need to improve the daily disinfection measures of air conditioning and ventilation equipment and cut off the transmission routes every quarter. It is more effective to control the sense of the hospital. (2) The comparison of resistance genes of resistance bacteria in various quarters of the hospital to the conventional culture specimens avoids the repeated detection of the same patient, and is more sensitive to the difficult culture bacteria than the conventional culture specimen. (3) Establish a resistance gene library of some drug-resistant bacteria in our hospital. In each quarter, each hospital department of pathology issued a microbiological room for different departments of drug resistant bacteria statistics. To increase the monitoring of drug resistance genes, improve the sensitivity of the original drug resistant bacteria monitoring, and reduce the biological pollution in the hospital environment can cause a series of epidemic outbreak of infectious diseases.